Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD70 antibody [EPR21946-290] - BSA and Azide free (ab239348)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21946-290] to CD70 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD70 antibody [EPR21946-290] - BSA and Azide free
    See all CD70 primary antibodies

  • Description

    Rabbit monoclonal [EPR21946-290] to CD70 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: A20 cells.

  • General notes

    Ab239348 is the carrier-free version of ab223292. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239348 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CD70 antibody [EPR21946-290] - BSA and Azide free (ab239348)
    Flow Cytometry - Anti-CD70 antibody [EPR21946-290] - BSA and Azide free (ab239348)

    Flow cytometric analysis of P815 (Mouse mastocytoma mast cell, left) and A20 (Mouse reticulum sarcoma cell line, right) cell lines labeling CD70 with ab223292 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2,000 dilution was used as the secondary antibody.

    Gated on viable cells.

    Negative control: P815.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223292).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD70 antibody [EPR21946-290] - BSA and Azide free (ab239348)
    Immunocytochemistry/ Immunofluorescence - Anti-CD70 antibody [EPR21946-290] - BSA and Azide free (ab239348)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A20 (Mouse reticulum sarcoma cell line) cells labeling CD70 with ab223292 at 1/2,000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green).

    Confocal image showing membranous staining in A20 cells with distinct staining of the target protein being transported from Golgi to cell surfaces (PMID: 20971706; PMID: 29496042).

    Negative control: P815 (PMID: 9620608).

    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The nuclear counter stain is DAPI (blue). The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab223292).

     

  • Anti-CD70 antibody [EPR21946-290] - BSA and Azide free (ab239348)
    Anti-CD70 antibody [EPR21946-290] - BSA and Azide free (ab239348)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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