Anti-CD70 antibody [EPR21946-290] (ab223292)
![Anti-CD70 antibody [EPR21946-290] (ab223292)](https://www.abcam.com/ps/products/223/ab223292/Images/ab223292-317504-anti-cd70-antibody-epr21946-290-immunofluorescence.jpg "Anti-CD70 antibody [EPR21946-290] (ab223292)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21946-290] to CD70
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-CD70 antibody [EPR21946-290]
See all CD70 primary antibodies -
Description
Rabbit monoclonal [EPR21946-290] to CD70 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: A20 cells. Flow Cyt: A20 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21946-290 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD70 antibody [EPR21946-290] (ab223292)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A20 (Mouse reticulum sarcoma cell line) cells labeling CD70 with ab223292 at 1/2,000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green).
Confocal image showing membranous staining in A20 cells with distinct staining of the target protein being transported from Golgi to cell surfaces (PMID: 20971706; PMID: 29496042).
Negative control: P815 (PMID: 9620608).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The nuclear counter stain is DAPI (blue). The negative control is the secondary antibody only.
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![Flow Cytometry - Anti-CD70 antibody [EPR21946-290] (ab223292)](https://www.abcam.com/ps/products/223/ab223292/Images/ab223292-317503-anti-cd70-antibody-epr21946-290-flow-cytometry.jpg)
Flow Cytometry - Anti-CD70 antibody [EPR21946-290] (ab223292)Flow cytometric analysis of P815 (Mouse mastocytoma mast cell, left) and A20 (Mouse reticulum sarcoma cell line, right) cell lines labeling CD70 with ab223292 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2,000 dilution was used as the secondary antibody.
Gated on viable cells.
Negative control: P815.
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![Anti-CD70 antibody [EPR21946-290] (ab223292)](https://www.abcam.com/ps/products/223/ab223292/Images/ab223292-3-benefits-of-recombinant-antibodies.png)
Anti-CD70 antibody [EPR21946-290] (ab223292)
