Anti-CD70 antibody [BU69] - BSA and Azide free (ab269573)
![Anti-CD70 antibody [BU69] - BSA and Azide free (ab269573)](https://www.abcam.com/ps/products/269/ab269573/Images/ab269573-368341-AntiCD70-antibody-BU69-BSA-Azide-free-flow-cytometry-raji-human.jpg "Anti-CD70 antibody [BU69] - BSA and Azide free (ab269573)")
Key features and details
- Mouse monoclonal [BU69] to CD70 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD70 antibody [BU69] - BSA and Azide free
See all CD70 primary antibodies -
Description
Mouse monoclonal [BU69] to CD70 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Tissue, cells or virus corresponding to CD70.
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Positive control
- Flow Cyt: Raji cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab269573 is the carrier-free version of ab77868. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
BU69 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-CD70 antibody [BU69] - BSA and Azide free (ab269573)
Overlay histograms showing left Raji and right SHSY5Y cells stained with ab77868 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab77868) (1x106 in 100µl at 1 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150177) was used at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable single cells.
This image was produced using the same antibody clone but in a different formulation ab77868, PBS and sodium azide.
