Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD70 antibody [BU69] - BSA and Azide free (ab269573)

Key features and details

  • Mouse monoclonal [BU69] to CD70 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD70 antibody [BU69] - BSA and Azide free
    See all CD70 primary antibodies

  • Description

    Mouse monoclonal [BU69] to CD70 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to CD70.

  • Positive control

    • Flow Cyt: Raji cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab269573 is the carrier-free version of ab77868. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Flow Cytometry - Anti-CD70 antibody [BU69] - BSA and Azide free (ab269573)
    Flow Cytometry - Anti-CD70 antibody [BU69] - BSA and Azide free (ab269573)

    Overlay histograms showing left Raji and right SHSY5Y cells stained with ab77868 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab77868) (1x106 in 100µl at 1 µg/ml) for 30 min on ice.

    The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150177) was used at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable single cells.

    This image was produced using the same antibody clone but in a different formulation ab77868, PBS and sodium azide.

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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