Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15780] to C6orf64 - C-terminal
- Suitable for: IHC-P, ICC/IF, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-C6orf64 antibody [EPR15780] - C-terminal
See all C6orf64 primary antibodies -
Description
Rabbit monoclonal [EPR15780] to C6orf64 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human testis, Human fetal liver, 293T and MCF7 cell lysates; Human kidney tissue; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15780 -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling C6orf64 with ab189918 at 1/50 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of paraformaldehyde-fixed HepG2 cells labeling C6orf64 with ab189918 at 1/100, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).
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All lanes : Anti-C6orf64 antibody [EPR15780] - C-terminal (ab189918) at 1/10000 dilution
Lane 1 : Human testis cell lysate
Lane 2 : Human fetal liver cell lysate
Lane 3 : 293T cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation of C6orf64 from Human fetal liver lysate using ab189918 at 1/50 or 1X PBS (negative control) (lane 2). Immunostained with ab189918 at 1/10000 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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