Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG) (ab278115)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.15 pg/ml
  • Range: 6.25 pg/ml - 400 pg/ml
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Myelin oligodendrocyte glycoprotein ELISA Kit (MOG)

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Extract 8 2.2%
    Inter-assay
    Sample n Mean SD CV%
    Extract 3 5.4%

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.15 pg/ml

  • Range

    6.25 pg/ml - 400 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 95 93% - 97%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human MOG ELISA kit (ab277115) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human MOG protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MOG with 1.15 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Myelin oligodendrocyte glycoprotein (MOG), a 16 kDa protein, mediates homophilic cell-cell adhesion. MOG is a component of the myelin sheath and may be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication. Research indicates MOG acts as a receptor for the rubella virus. MOG shares 86%, 86%, 91%, and 98% sequence homology with mouse, rat, cow, and monkey, respectively.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Myelin oligodendrocyte glycoprotein (MOG) Capture Antibody 1 x 600µl
    10X Human Myelin oligodendrocyte glycoprotein (MOG) Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BI 1 x 6ml
    Human Myelin oligodendrocyte glycoprotein (MOG) Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.

  • Tissue specificity

    Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Alternative names

    • BTN6
    • BTNL11
    • MGC26137
    • MOG
    • MOG alpha 5
    • MOG alpha 6
    • MOG AluA
    • MOG AluB
    • MOG Ig AluB
    • MOG_HUMAN
    • MOGIG2
    • Myelin oligodendrocyte glycoprotein
    • Myelin-oligodendrocyte glycoprotein
    • NRCLP7
    see all

  • Database links

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Recombinant Antibody benefits
      Recombinant Antibody benefits

      To learn more about the advantages of recombinant antibodies see here.

    • Example of human Myelin oligodendrocyte glycoprotein standard curve in 1X Cell Extraction Buffer PTR.
      Example of human Myelin oligodendrocyte glycoprotein standard curve in 1X Cell Extraction Buffer PTR.

      The Myelin oligodendrocyte glycoprotein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native Myelin oligodendrocyte glycoprotein in human brain based on a 500 ng/mL extract load.
      Interpolated concentrations of native Myelin oligodendrocyte glycoprotein in human brain based on a 500 ng/mL extract load.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    © 2021 Astana Biomed Group LLP. All rights reserved.