Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20244] to NG2
Suitable for: WB, ICC/IF, IHC-P
Reacts with: Human

Product name

Anti-NG2 antibody [EPR20244]
See all NG2 primary antibodies
Description

Rabbit monoclonal [EPR20244] to NG2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A-375 and MeWo whole cell lysates; Human skin lysate. IHC-P: Human melanoma and glioma tissues. ICC/IF: A-375 and MeWo cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20244
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR20244] (ab183929)

Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling NG2 with ab183929 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and weak cytoplasmic staining on tumor cells of human melanoma (PMID: 24258997). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-NG2 antibody [EPR20244] (ab183929)

All lanes : Anti-NG2 antibody [EPR20244] (ab183929) at 1/1000 dilution

Lane 1 : A-375 (human malignant melanoma cell line), whole cell lysate
Lane 2 : Human skin lysate
Lane 3 : MeWo (human malignant melanoma cell line), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1 & 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 251 kDa
Observed band size: 270 kDa
why is the actual band size different from the predicted?

Exposure times: Lanes 1,3: 1 minute; Lane 2: 3 minutes.

Blocking/Dilution buffer: 5% NFDM/TBST.

This image of lane 3 was performed on a BIO-RAD® ChemiDoc™ MP instrument.
Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR20244] (ab183929)

Immunofluorescent analysis of 100% methanol-fixed A-375 (human malignant melanoma cell line) cells labeling NG2 with ab183929 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membrane staining on A-375 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR20244] (ab183929)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling NG2 with ab183929 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on blood vessels of human glioma (PMID: 24386429). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR20244] (ab183929)

Immunofluorescent analysis of 100% methanol-fixed MeWo (human malignant melanoma cell line) cells labeling NG2 with ab183929 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membrane staining on MeWo cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-NG2 antibody [EPR20244] (ab183929)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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