Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14773] to alpha Sarcoglycan
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-alpha Sarcoglycan antibody [EPR14773]
    See all alpha Sarcoglycan primary antibodies

  • Description

    Rabbit monoclonal [EPR14773] to alpha Sarcoglycan

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skeletal muscle, mouse heart and rat heart lysates; Human skeletal muscle tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14773

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)
    Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)
    Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)
    Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)
    All lanes : Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)

    Immunhistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling alpha Sarcoglycan with ab189254 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)
    Immunoprecipitation - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)

    Immunoprecipitation analysis of human skeletal muscle tissue lysate labeling alpha Sarcoglycan using ab189254 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of human skeletal muscle tissue lysate.

  • Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)
    Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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