Anti-NG2 antibody [132.38] (ab50009)
![Anti-NG2 antibody [132.38] (ab50009)](https://www.abcam.com/ps/products/50/ab50009/Images/ab50009-215950-anti-ng2-antibody-13238-western-blot.jpg "Anti-NG2 antibody [132.38] (ab50009)")
Key features and details
- Mouse monoclonal [132.38] to NG2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-NG2 antibody [132.38]
See all NG2 primary antibodies -
Description
Mouse monoclonal [132.38] to NG2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Does not react with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Rat NG2.
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Positive control
- WB: Rat brain, spinal cord and hippocampus tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Primary antibody notes
This antibody is derived from the hybridoma 132.38 produced by the fusion of mouse myeloma cells (P3XAg8.653) and splenocytes from BALB/c mice. -
Clonality
Monoclonal -
Clone number
132.38 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-NG2 antibody [132.38] (ab50009)All lanes : Anti-NG2 antibody [132.38] (ab50009) at 1 µg/ml
Lane 1 : Rat Brain Tissue Lysate
Lane 2 : Rat Spinal Cord Tissue Lysate
Lane 3 : Rat Hippocampus Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 251 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa (possible non-specific binding), 41 kDa (possible non-specific binding)
Exposure time: 20 minutes
