Anti-Caspr2/CNTNAP2 antibody (ab153856)
Key features and details
- Rabbit polyclonal to Caspr2/CNTNAP2
- Suitable for: WB, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Caspr2/CNTNAP2 antibody
See all Caspr2/CNTNAP2 primary antibodies -
Description
Rabbit polyclonal to Caspr2/CNTNAP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Caspr2/CNTNAP2 aa 287-633.
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Positive control
- WB: Molt-4, and mouse brain lysates; ICC/IF: HeLa cells and DIV14 rat E18 primary cortical neurons . IHC-Fr: E13.5 Rat brain and embryonic mouse brain (mE18.5).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (ab153856)
DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min staining Caspr2/CNTNAP2 protein by immunofluorescent analysis (1:500) (green).
Counter stained using beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500 (red) and DAPI (blue). -
Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (ab153856)Frozen section of embryonic mouse brain (mE18.5) staining Caspr2/CNTNAP2 proteins in embryonic mouse brain by immunohistochemical analysis.
Red: ab153856 diluted at 1:250.
Blue: DAPI. -
Immunohistochemistry (Frozen sections) - Anti-Caspr2/CNTNAP2 antibody (ab153856)Frozen sectioned E13.5 Rat brain staining Caspr2/CNTNAP2 protein expression by immunohistochemical analysis.
Green: ab153856 diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500.
Blue: Fluoroshield with DAPI. -
Western blot - Anti-Caspr2/CNTNAP2 antibody (ab153856)Anti-Caspr2/CNTNAP2 antibody (ab153856) at 1/5000 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size: 148 kDa
5% SDS PAGE -
Western blot - Anti-Caspr2/CNTNAP2 antibody (ab153856)Anti-Caspr2/CNTNAP2 antibody (ab153856) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 148 kDa
5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Caspr2/CNTNAP2 antibody (ab153856)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Caspr2/CNTNAP2 with ab153856 at 1/200 dilution. Lower panel is merged with DNA probe.
