Anti-NM23A antibody [EPR10146] - BSA and Azide free (ab249655)
![Anti-NM23A antibody [EPR10146] - BSA and Azide free (ab249655)](https://www.abcam.com/ps/products/249/ab249655/Images/ab249655-397730-anti-nm23a-antibody-epr10146-western-blot-human-knockout-lysate.jpg "Anti-NM23A antibody [EPR10146] - BSA and Azide free (ab249655)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10146] to NM23A - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-NM23A antibody [EPR10146] - BSA and Azide free
See all NM23A primary antibodies -
Description
Rabbit monoclonal [EPR10146] to NM23A - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment within Human NM23A. The exact sequence is proprietary.
Database link: P15531 -
Positive control
- WB: HEK293T, MCF7 and Jurkat cell lysates.
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General notes
Ab249655 is the carrier-free version of ab171935. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249655 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10146 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NM23A antibody [EPR10146] - BSA and Azide free (ab249655)All lanes : Anti-NM23A antibody [EPR10146] (ab171935) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : NME1 knockout HEK293T cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab171935).
Lanes 1-4: Merged signal (red and green). Green - ab171935 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa.
ab171935 Anti-NM23A antibody [EPR10146] was shown to specifically react with NM23A in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266215 (knockout cell lysate ab258075) was used. Wild-type and NM23A knockout samples were subjected to SDS-PAGE. ab171935 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NM23A antibody [EPR10146] - BSA and Azide free (ab249655)](https://www.abcam.com/ps/products/249/ab249655/Images/ab249655-398728-anti-nm23a-antibody-epr10146-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-NM23A antibody [EPR10146] - BSA and Azide free (ab249655)All lanes : Anti-NM23A antibody [EPR10146] (ab171935) at 1/1000 dilution
Lane 1 : HeLa cell line lysate
Lane 2 : MCF7 cell line lysate
Lane 3 : Jurkat cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat ant-rabbit HRP conjugated antibody at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 17 kDaThis data was developed using ab171935, the same antibody clone in a different buffer formulation.
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![Anti-NM23A antibody [EPR10146] - BSA and Azide free (ab249655)](https://www.abcam.com/ps/products/249/ab249655/Images/ab249655-3-benefits-of-recombinant-antibodies.png)
Anti-NM23A antibody [EPR10146] - BSA and Azide free (ab249655)
