Anti-Beclin 1 antibody [2A4] (ab114071)
![Anti-Beclin 1 antibody [2A4] (ab114071)](https://www.abcam.com/ps/products/114/ab114071/Images/ab114071-126956-anti-beclin-1-antibody-2a4-western-blot.jpg "Anti-Beclin 1 antibody [2A4] (ab114071)")
Key features and details
- Mouse monoclonal [2A4] to Beclin 1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Beclin 1 antibody [2A4]
See all Beclin 1 primary antibodies -
Description
Mouse monoclonal [2A4] to Beclin 1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Beclin 1 aa 51-248.
Database link: Q14457 -
Positive control
- WB: Hela; A431; MCF7; RAJI; Jurkat and SKBR3 cell lysates IHC-P: Breast cancer and liver cancer tissues
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2A4 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Beclin 1 antibody [2A4] (ab114071)All lanes : Anti-Beclin 1 antibody [2A4] (ab114071) at 1/500 dilution
Lane 1 : Hela cell lysate
Lane 2 : A431 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Raji cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : SKBR3 cell lysate
Predicted band size: 60 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [2A4] (ab114071)](https://www.abcam.com/ps/products/114/ab114071/Images/ab114071-126955-anti-beclin-1-antibody-2a4-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [2A4] (ab114071)Immunohistochemical analysis of Beclin 1 expression in paraffin embedded breast cancer tissues (left) and liver cancer tissues (right) using ab114071 at 1/200 dilution with DAB staining -
![Flow Cytometry - Anti-Beclin 1 antibody [2A4] (ab114071)](https://www.abcam.com/ps/products/114/ab114071/Images/ab114071-126954-anti-beclin-1-antibody-2a4-flow-cytometry.png)
Flow Cytometry - Anti-Beclin 1 antibody [2A4] (ab114071)Flow cytometric analysis of RAJI cells using Beclin 1 mouse mAb (green) and negative control (purple).
