Anti-Beclin 1 antibody (ab92389)
Key features and details
- Rabbit polyclonal to Beclin 1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Beclin 1 antibody
See all Beclin 1 primary antibodies -
Description
Rabbit polyclonal to Beclin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human Beclin 1 (NP_003757.1)
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Positive control
- HeLa, 293T or NIH3T3 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Beclin 1 antibody (ab92389)All lanes : Anti-Beclin 1 antibody (ab92389)
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-Beclin 1 antibody (ab92389)Sample: HeLa whole cell lysate (1mg for IP, 20% of IP loaded).
Antibodies: ab92389 used for WB 1µg/ml (lane 1) and used for IP at 3µg/mg lysate. Lane 2: control IgG.
Detection: chemiluminescence with exposure time of 10 seconds.
