Human Free Estriol ELISA Kit (ab108671)
Key features and details
- Sensitivity: 0.05 ng/ml
- Range: 0.1 ng/ml - 12 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Human Free Estriol ELISA Kit
See all Estriol kits -
Detection method
Colorimetric -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
= 0.05 ng/ml -
Range
0.1 ng/ml - 12 ng/ml -
Recovery
101.6 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Free Estriol competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Free Estriol in serum and plasma.
A 96-well plate has been precoated with anti-Estriol IgG. Samples and the Estriol-HRP conjugate are added to the wells, where Estriol in the sample competes with the added Estriol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Solution 1 x 50ml anti-Estriol IgG Coated Microplate (12 x 8 wells) 1 unit Estriol Control 1 x 1ml Estriol HRP Conjugate 1 x 15ml Estriol Standard 0 ng/mL 1 x 1ml Estriol Standard 0.15 ng/mL 1 x 1ml Estriol Standard 0.75 ng/mL 1 x 1ml Estriol Standard 2.0 ng/mL 1 x 1ml Estriol Standard 5.0 ng/ml 1 x 1ml Estriol Standard 15.0 ng/mL 1 x 1ml Estriol Standard 40.0 ng/ml 1 x 1ml Stop Solution 1 x 15ml TMB Substrate Solution 1 x 15ml -
Research areas
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Alternative names
- Oestriol
Images
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Typical Stanard Curve
Representative Standard Curve using ab108671.