Estriol ELISA Kit (ab108675)
Key features and details
- Sensitivity: 0.22 ng/ml
- Range: 2 ng/ml - 200 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
Estriol ELISA Kit
See all Estriol kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Control Sera 32 Inter-assay Sample n Mean SD CV% Control Sera 36 -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
0.22 ng/ml -
Range
2 ng/ml - 200 ng/ml -
Recovery
94.88 %
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Assay duration
Multiple steps standard assay -
Product overview
Abcam’s Estriol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estriol in serum and plasma.
A 96-well plate has been precoated with anti-Estriol. Samples and the Estriol-HRP conjugate are added to the wells, where Estriol in the sample competes with the added Estriol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Anti-Estriol IgG Coated Microplate (12 x 8 wells) 1 unit Control A 1 x 1ml Control B 1 x 1ml Cover foils 1 unit Estriol Standard 0 - 0 ng/mL 1 x 1ml Estriol Standard 1 – 2 ng/mL 1 x 1ml Estriol Standard 2 – 20 ng/mL 1 x 1ml Estriol Standard 3 – 80 ng/mL 1 x 1ml Estriol Standard 4 – 200 ng/mL 1 x 1ml Estriol-HRP Conjugate 1 x 22ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml Wash Solution 1 x 50ml -
Research areas
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Alternative names
- Oestriol
