Estriol ELISA Kit (ab178662)
Key features and details
- Sensitivity: 1 pg/ml
- Sample type: Saliva
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Estriol ELISA Kit
See all Estriol kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Saliva 16 Inter-assay Sample n Mean SD CV% Saliva 10 -
Sample type
Saliva -
Assay type
Competitive -
Sensitivity
1 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Spike 104 50pg/ml - 300pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Estriol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estriol in saliva.
A 96-well plate has been precoated with anti-Estriol antibodies (solid-phase). Samples and the Estriol HRP Conjugate are added to the wells, where Estriol in the sample competes with the added Estriol HRP Conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estriol in the sample and the intensity is measured at 450 nm. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 50X Washing Solution 1 x 20ml anti-Estriol IgG Coated Microplate (12 x 8 wells) 1 unit Cover Foil 1 unit Estriol HRP Conjugate 1 x 1ml Estriol Low Control 1 x 1ml Estriol Control 1 x 1ml Estriol Standard 0 - 0 pg/mL 1 x 1ml Estriol Standard 1 - 2.5 pg/mL 1 x 1ml Estriol Standard 2 - 15 pg/mL 1 x 1ml Estriol Standard 3 - 100 pg/mL 1 x 1ml Estriol Standard 4 - 600 pg/mL 1 x 1ml Estriol Standard 5 - 4,000 pg/mL 1 x 1ml Incubation Buffer 1 x 30ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
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Alternative names
- Oestriol
