Recombinant rat PDE4B protein (ab198434)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant rat PDE4B protein
See all PDE4B proteins and peptides -
Biological activity
15,345 pmole/min/µg. Assay Description: One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of rPDE4B at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
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Purity
>= 70 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDPLVPRGSPGEFTAKNSSKELPASESEVCIK TFKEQMRLELELPKLPGNRPTSPKISPRSSPRNSPCFFRKLLVNKSIRQR RRFTVAHTCFDVENGPSPGRSPLDPQASSSSGLVLHAAFPGHSQRRESFL YRSDSDYDLSPKAMSRNSSLPSEQHGDDLIVTPFAQVLASLRSVRNNFTL LTNLHGAPNKRSPAASQAPVTRVSLQEESYQKLAMETLEELDWCLDQLET IQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQND VEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLNNTSISRFGVNTENE DHLAKELEDLNKWGLNIFNVAGYSHNRPLTCIMYAIFQERDLLKTFKISS DTFVTYMMTLEDHYHSDVAYHNSLHAADVAQSTHVLLSTPALDAVFTDLE ILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFK LLQEEHCDIFQNLTKKQRQTLRKMVIDMVLATDMSKHMSLLADLKTMVET KKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIMEE FFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQ PDAQDILDTLEDNRNWYQSMIPQSPSPPLDERSRDCQGLMEKFQFELTLE EEDSEGPEKEGEGPNYFSSTKTLCKPCHAANGLALPVGGGNAASTQPRCG HV -
Predicted molecular weight
108 kDa including tags -
Amino acids
2 to 722 -
Tags
GST tag N-Terminus -
Additional sequence information
(NM_017031)
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.64% Tris HCl, 0.64% Sodium chloride, 0.017% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant rat PDE4B protein (ab198434)
Activity assay of ab198434.
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SDS-PAGE - Recombinant rat PDE4B protein (ab198434)4-20% SDS-PAGE analysis of 2 µg ab198434 with Coomassie staining.
