Recombinant dog PDE4B protein (ab198483)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 50% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant dog PDE4B protein
See all PDE4B proteins and peptides -
Biological activity
Specific activity: ≥ 3882 pmol/min/µg
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198483 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
Purity
>= 50 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Dog -
Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFKDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDH LVPRGSGSGIQKKSRSVMAVTADDN VKDYLE CSLSKSYSSSSNTLGIDLWRGRRCCSGNLQLPPLAQRQSERARTPEGDGI SRPTTLPLMTLPSIAITTVSQECFDVENGPSPGRSPLDPQASSSSGLVLH ATFPGHSQRRESFLYRSDSDYDLSPKAMSRNSSLPSEQHGDDLIVTPFAQ VLASLRSVRNNFTVLTNLHGASNKRSPAATSQPPVSRVNLQEESYQKLAM ETLEELDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQ VSEYISNTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSL NNTSISRFGVNTENEDHLAKELEDLNKWGLNIFNVAGYSHNRPLTCIMYA IFQERDLLKTFKISSDTFVTYMMTLEDHYHSDVAYHNSLHAADVAQSTHV LLSTPALDAVFTDLEILATIFAAAIHDVDHPGVSNQFLINTNSELALMYN DESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQTLRKMVIDMVLATDMS KHMSLLADLKTMVETKKVTSSGVLL LDNYTDRIQVLRNMVHCADLSNP TKSLELYRQWTDRIMEEFFQQGDKERER GMEISPMCDKHTASVEKSQV GFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPPLDE QNRDCQGLMEKFQFELTLEEEDSEGPDKEGEGHNYFSSTKTLCVIDPEHR DSLGDTDGDTGAENKTPID -
Predicted molecular weight
110 kDa including tags -
Amino acids
2 to 737 -
Tags
GST tag N-Terminus
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Preparation and Storage
- 5''-cyclic phosphodiesterase 4B
- cAMP-specific 3''
- cAMP-specific 3',5'-cyclic phosphodiesterase 4B
Images
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Functional Studies - Recombinant dog PDE4B protein (ab198483)
Specific activity analysis of ab198483.
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SDS-PAGE - Recombinant dog PDE4B protein (ab198483)4-20% SDS PAGE analysis of ab198483 (2 μg).
