Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NM23A antibody [EPR3036] (ab92327)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3036] to NM23A
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-NM23A antibody [EPR3036]
    See all NM23A primary antibodies

  • Description

    Rabbit monoclonal [EPR3036] to NM23A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NM23A aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: HEK293T, MCF7, A375, HeLa and Jurkat whole cell lysate (ab7899) IHC-P: Human lung adenocarcinoma tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3036

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-NM23A antibody [EPR3036] (ab92327)
    Western blot - Anti-NM23A antibody [EPR3036] (ab92327)
    All lanes : Anti-NM23A antibody [EPR3036] (ab92327) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : NME1 knockout HEK293T cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab92327 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab92327 Anti-NM23A antibody [EPR3036] was shown to specifically react with NM23A in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266215 (knockout cell lysate ab258075) was used. Wild-type and NM23A knockout samples were subjected to SDS-PAGE. ab92327 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-NM23A antibody [EPR3036] (ab92327)
    Flow Cytometry - Anti-NM23A antibody [EPR3036] (ab92327)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NM23A with unpurified ab92327 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NM23A antibody [EPR3036] (ab92327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NM23A antibody [EPR3036] (ab92327)

    ab92327, at a dilution of 1/100, staining NM23A in formalin fixed, paraffin embedded Human lung adenocarcinoma tissue by Immunohistochemistry. Detection: DAB staining

    Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • Western blot - Anti-NM23A antibody [EPR3036] (ab92327)
    Western blot - Anti-NM23A antibody [EPR3036] (ab92327)
    All lanes : Anti-NM23A antibody [EPR3036] (ab92327) at 1/5000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : A375 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 17 kDa

  • Anti-NM23A antibody [EPR3036] (ab92327)
    Anti-NM23A antibody [EPR3036] (ab92327)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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