Anti-Beclin 1 antibody (ab217179)
Key features and details
- Rabbit polyclonal to Beclin 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Beclin 1 antibody
See all Beclin 1 primary antibodies -
Description
Rabbit polyclonal to Beclin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Beclin 1 aa 200-300 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q14457 -
Positive control
- Mouse intestine lysates; rat kidney, rat brain and rat stomach tissues; 293T lysates; U138 and SMMC7721 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine)
Aqueous buffered solution -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Beclin 1 antibody (ab217179)Anti-Beclin 1 antibody (ab217179) at 1/300 dilution + 293T lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution
Predicted band size: 52 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (ab217179)Immunofluorescent analysis of SMMC7721 cells labeling Beclin 1 with ab217179 at 1/50 dilution followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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Western blot - Anti-Beclin 1 antibody (ab217179)All lanes : Anti-Beclin 1 antibody (ab217179) at 1/200 dilution
Lane 1 : mouse brain lysates
Lane 2 : mouse intestine lysates
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 52 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (ab217179)Immunofluorescent analysis of U138 cells labeling Beclin 1 with ab217179 at 1/100 dilution followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab217179)Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat stomach tissue labeling Beclin 1 with ab217179 at 1/200 dilution, followed by conjugation to the secondary antibody (Goat Anti-Rabbit IgG, Cy3 conjugated) at 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab217179)Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling Beclin 1 with ab217179 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab217179)Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat kidney tissue labeling Beclin 1 with ab217179 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
