Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aggrecan antibody [6-B-4] (ab3778)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [6-B-4] to Aggrecan
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Aggrecan antibody [6-B-4]
    See all Aggrecan primary antibodies

  • Description

    Mouse monoclonal [6-B-4] to Aggrecan

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Recombinant human Aggrecan protein. IHC-P: Human cartilage sections.

  • General notes

    This product has switched from a hybridoma to recombinant production method on 08th October 2020.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59.04% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Primary antibody notes

    This antibody detects aggrecan metabolites (intact or matrix protease-catabolised) in human synovial fluid samples.

  • Clonality

    Monoclonal

  • Clone number

    6-B-4

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)
    Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)

    ab3778 was shown to react with recombinant human Aggrecan protein in western blot. 

    Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3778 at 1µg/ml overnight at 4°C. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye®800CW) preabsorbed (ab216772) at a 1/20000 dilution for 1 hour at RT.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)

    Secreted staining of cartilage cells.

    Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)

    No staining in negative control tissue (human normal liver).

    Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Anti-Aggrecan antibody [6-B-4] (ab3778)
    Anti-Aggrecan antibody [6-B-4] (ab3778)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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