Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [6-B-4] to Aggrecan
Suitable for: WB, IHC-P
Reacts with: Human, Recombinant fragment

Product name

Anti-Aggrecan antibody [6-B-4]
See all Aggrecan primary antibodies
Description

Mouse monoclonal [6-B-4] to Aggrecan
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Recombinant fragment

See all applications and species data
Immunogen

Synthetic peptide. This information is considered to be commercially sensitive.
Positive control
- WB: Recombinant human Aggrecan protein. IHC-P: Human cartilage sections.
General notes
This product has switched from a hybridoma to recombinant production method on 08^th October 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IHC-P	Human
WB	Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59.04% PBS
Concentration information loading...
Purity

Protein A purified
Primary antibody notes

This antibody detects aggrecan metabolites (intact or matrix protease-catabolised) in human synovial fluid samples.
Clonality

Monoclonal
Clone number

6-B-4
Isotype

IgG1
Light chain type

kappa
Research areas

Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)

ab3778 was shown to react with recombinant human Aggrecan protein in western blot.

Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3778 at 1µg/ml overnight at 4°C. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye®800CW) preabsorbed (ab216772) at a 1/20000 dilution for 1 hour at RT.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)

Secreted staining of cartilage cells.

Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)

No staining in negative control tissue (human normal liver).

Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Anti-Aggrecan antibody [6-B-4] (ab3778)

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