Anti-Aggrecan antibody (ab36861)
Key features and details
- Rabbit polyclonal to Aggrecan
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Aggrecan antibody
See all Aggrecan primary antibodies -
Description
Rabbit polyclonal to Aggrecan -
Host species
Rabbit -
Specificity
This antibody detects recombinant human Aggrecan by Western Blotting. We have been unable to detect endogenous protein in WB with ab36861. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human, Recombinant fragment
Predicted to work with: Dog
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Immunogen
Synthetic peptide corresponding to Rat Aggrecan aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab36860) -
Positive control
- WB: Aggrecan G1-IGD-G2 recombinant protein. IHC-P: Rat liver tissue, human liver tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab36861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 250 kDa). This application has only been tested on recombinant protein.
Target
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Relevance
Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS. -
Cellular localization
Secreted; extracellular matrix. -
Database links
- Entrez Gene: 176 Human
- Entrez Gene: 58968 Rat
- Omim: 155760 Human
- SwissProt: Q28343 Dog
- SwissProt: P16112 Human
- SwissProt: P07897 Rat
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Alternative names
- ACAN antibody
- AGC 1 antibody
- AGC1 antibody
see all
Images
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Western blot - Anti-Aggrecan antibody (ab36861)Anti-Aggrecan antibody (ab36861) at 1 µg/ml + Aggrecan G1-IGD-G2 recombinant protein
Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 250 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?This antibody was tested on Human Aggrecan G1-IGD-G2 Domains recombinant protein which migrates at approximately 120 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody (ab36861)IHC image of Aggrecan staining in rat liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36861, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody (ab36861)IHC image of Aggrecan staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36861, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References (76)
ab36861 has been referenced in 76 publications.
- Ding SL et al. Urolithin a attenuates IL-1ß-induced inflammatory responses and cartilage degradation via inhibiting the MAPK/NF-?B signaling pathways in rat articular chondrocytes. J Inflamm (Lond) 17:13 (2020). PubMed: 32210738
- Chen S et al. Moderate Fluid Shear Stress Regulates Heme Oxygenase-1 Expression to Promote Autophagy and ECM Homeostasis in the Nucleus Pulposus Cells. Front Cell Dev Biol 8:127 (2020). PubMed: 32195253
- Bao J et al. Rapamycin protects chondrocytes against IL-18-induced apoptosis and ameliorates rat osteoarthritis. Aging (Albany NY) 12:5152-5167 (2020). PubMed: 32182210
- Lei C et al. Influences of circulatory factors on intervertebral disc aging phenotype. Aging (Albany NY) 12:12285-12304 (2020). PubMed: 32527988
- Liu N et al. MicroRNA-181 regulates the development of Ossification of Posterior longitudinal ligament via Epigenetic Modulation by targeting PBX1. Theranostics 10:7492-7509 (2020). PubMed: 32685001
Images
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Western blot - Anti-Aggrecan antibody (ab36861)Anti-Aggrecan antibody (ab36861) at 1 µg/ml + Aggrecan G1-IGD-G2 recombinant protein
Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 250 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?This antibody was tested on Human Aggrecan G1-IGD-G2 Domains recombinant protein which migrates at approximately 120 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody (ab36861)
IHC image of Aggrecan staining in rat liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36861, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody (ab36861)IHC image of Aggrecan staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36861, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
