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Immunology Adaptive Immunity T Cells CD

Anti-CD272/BTLA antibody [7B8B4] (ab233733)

Anti-CD272/BTLA antibody [7B8B4] (ab233733)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [7B8B4] to CD272/BTLA
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CD272/BTLA antibody [7B8B4]
    See all CD272/BTLA primary antibodies
  • Description

    Mouse monoclonal [7B8B4] to CD272/BTLA
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CD272/BTLA aa 31-157. (Expressed in E.coli).
    Database link: Q7Z6A9

  • Positive control

    • WB: Recombinant human CD272/BTLA (aa 31-157) protein; CD272/BTLA (aa 31-157)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as CD272

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    7B8B4
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD

Images

  • Western blot - Anti-CD272/BTLA antibody [7B8B4] (ab233733)
    Western blot - Anti-CD272/BTLA antibody [7B8B4] (ab233733)
    Anti-CD272/BTLA antibody [7B8B4] (ab233733) at 1/500 dilution + Recombinant human CD272 (aa 31-157) protein

    Predicted band size: 33 kDa



    Expected MW is 41 kDa.

  • Western blot - Anti-CD272/BTLA antibody [7B8B4] (ab233733)
    Western blot - Anti-CD272/BTLA antibody [7B8B4] (ab233733)
    All lanes : Anti-CD272/BTLA antibody [7B8B4] (ab233733) at 1/500 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : CD272/BTLA (aa 31-157)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 33 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CD272/BTLA antibody [7B8B4] (ab233733)
    Immunocytochemistry/ Immunofluorescence - Anti-CD272/BTLA antibody [7B8B4] (ab233733)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD272/BTLA (green) using ab233733 at 1/200 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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