Mouse Neprilysin ELISA Kit (ab267581)
Key features and details
- Sensitivity: 61 pg/ml
- Range: 61.44 pg/ml - 15000 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Neprilysin ELISA Kit
See all CD10 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
61 pg/ml -
Range
61.44 pg/ml - 15000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 77 70% - 105% Plasma 89 77% - 99% Cell culture media 108 93% - 119% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Neprilysin ELISA Kit is designed for the quantitative determination of Neprilysin in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for mouse Neprilysin coated on a 96-well plate. Standards and samples are pipetted into the wells and Neprilysin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Neprilysin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neprilysin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP1alpha, MIP-1gamma, MIP-2, MIP-3beta, MIP-3alpha, PF-4, P-Selectin, RANTES, SCF, SDF1alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Mouse Neprilysin coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Mouse Neprilysin Detection Antibody 2 vials Mouse Neprilysin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. -
Sequence similarities
Belongs to the peptidase M13 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Atriopeptidase
- CALLA
- CD10
see all -
Database links
- Entrez Gene: 17380 Mouse
- SwissProt: Q61391 Mouse
- Unigene: 296022 Mouse
Images
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Standard curve
Mouse Neprilysin ELISA kit (ab267581) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.