Anti-ADAMTS5 antibody - Carboxyterminal end (ab39202)
Key features and details
- Rabbit polyclonal to ADAMTS5 - Carboxyterminal end
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADAMTS5 antibody - Carboxyterminal end
See all ADAMTS5 primary antibodies -
Description
Rabbit polyclonal to ADAMTS5 - Carboxyterminal end -
Host species
Rabbit -
Specificity
ab39202 recognises the Carboxyterminal end of ADAMTS5. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide crresponding to aa 850-890 of the carboxyterminal end of human ADAMTS5.
Read Abcam's proprietary immunogen policy (Peptide available as ab43061.) -
Positive control
- IHC-P: Human normal liver parenchym
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody - Carboxyterminal end (ab39202)Ab39202 staining Human normal liver parenchym. Staining is localized to cytoplasma and secreted.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
