Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Versican antibody [EPR23374-151] (ab270445)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23374-151] to Versican
Suitable for: WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-Versican antibody [EPR23374-151]
See all Versican primary antibodies
Description

Rabbit monoclonal [EPR23374-151] to Versican
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain, Human brain lysates. IHC-P: Human cerebrum, Human testis and Human stomach, Mouse cerebrum tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23374-151
Isotype

IgG
Research areas

Western blot - Anti-Versican antibody [EPR23374-151] (ab270445)

All lanes : Anti-Versican antibody [EPR23374-151] (ab270445) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MeWo (human malignant melanoma fibroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 373 kDa
Observed band size: 370 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: MDA-MB-231(PMID:29222454); MeWo(PMID:12972299).

Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23374-151] (ab270445)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Versican with ab270445 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum (PMID: 18936977). The section was incubated with ab270445 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23374-151] (ab270445)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Versican with ab270445 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in stromal cells of human stomach (PMID: 26619403). The section was incubated with ab270445 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23374-151] (ab270445)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Versican with ab270445 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in tunica propria of human seminiferous tubules (PMID: 8601689). The section was incubated with ab270445 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Versican antibody [EPR23374-151] (ab270445)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Versican with ab270445 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum. The section was incubated with ab270445 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Anti-Versican antibody [EPR23374-151] (ab270445)

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