Anti-Versican antibody [EPR23374-21] - BSA and Azide free (ab272223)
![Anti-Versican antibody [EPR23374-21] - BSA and Azide free (ab272223)](https://www.abcam.com/ps/products/272/ab272223/Images/ab272223-387966-antiversicanantibodyepr2337421westernblotmousebrainhumanbrainmdamb231meworatbrainlysates.jpg "Anti-Versican antibody [EPR23374-21] - BSA and Azide free (ab272223)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23374-21] to Versican - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Versican antibody [EPR23374-21] - BSA and Azide free
See all Versican primary antibodies -
Description
Rabbit monoclonal [EPR23374-21] to Versican - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain, Human brain, Rat brain lysates.
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General notes
ab272223 is the carrier-free version of ab270444. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23374-21 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Versican antibody [EPR23374-21] - BSA and Azide free (ab272223)All lanes : Anti-Versican antibody [EPR23374-21] (ab270444) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MeWo (human malignant melanoma fibroblast) whole cell lysate
Lane 5 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 373 kDa
Observed band size: 370 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: MDA-MB-231(PMID:29222454); MeWo(PMID:12972299).
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containg PBS, glycerol, BSA and sodium azide (ab270444).
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![Anti-Versican antibody [EPR23374-21] - BSA and Azide free (ab272223)](https://www.abcam.com/ps/products/272/ab272223/Images/ab272223-2-benefits-of-recombinant-antibodies.png)
Anti-Versican antibody [EPR23374-21] - BSA and Azide free (ab272223)
