Mouse Versican ELISA Kit (ab279409)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Versican ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 10 3.4% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
12 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 92 89% - 95% Serum 102 96% - 109% Hep Plasma 92 87% - 99% Cit plasma 95 90% - 104% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Versican ELISA kit (ab279409) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Versican protein in mouse serum, plasma cell culture supernatatn samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Versican with 12 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Versican, also known as Versican Core Protein and Chondroitin Sulfate Proteoglycan Core Protein 2, is secreted protein that binds hyaluronic acid. It may play a role in intercellular signaling and in connecting cells with the extracellular matrix. Versican may take part in the regulation of cell motility, growth and differentiation.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Versican Capture Antibody 1 x 600µl 10X Mouse Versican Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse Versican Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 100ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. -
Tissue specificity
Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas. -
Involvement in disease
Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN. -
Sequence similarities
Belongs to the aggrecan/versican proteoglycan family.
Contains 1 C-type lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain. -
Developmental stage
Disappears after the cartilage development. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Chondroitin sulfate proteoglycan 2
- Chondroitin sulfate proteoglycan core protein 2
- Chondroitin sulfate proteoglycan core protein, cartilage
see all -
Database links
- Entrez Gene: 13003 Mouse
- SwissProt: Q62059 Mouse
- Unigene: 158700 Mouse
- Unigene: 410783 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of mouse Versican standard curve in Sample Diluent NS.The Versican standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Versican in mouse serum, plasma and heart supernatant samples.The concentrations of Versican were measured in duplicates, interpolated from the Versican standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 15%, plasma (citrate) 3.75%, plasma (heparin) 3.75% and heart supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Versican concentration was determined to be 44.9 ng/mL in neat serum, 49.1 ng/mL in neat plasma (citrate), 41.1 ng/mL in neat plasma (heparin) and 28.5 ng/mL in neat heart supernatant.