Recombinant human Cyclin E1 + Cdk3 protein (Active) (ab196131)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human Cyclin E1 + Cdk3 protein (Active) -
Biological activity
Specific activity of ab196131 was determined to be 30 pmol/min/μg. Assay Conditions: Kinase buffer containing 1 mM DTT using Histone H1 (0.1 mg/mL) substrate and 20 µM ATP at 30°C for 50 min.
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Purity
> 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Predicted molecular weight
60 kDa -
Tags
GST tag N-Terminus -
Additional sequence information
Recombinant full length human Cdk3 (NM_001258)
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Amino Acid Sequence 2
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Species
Human -
Predicted molecular weight
73 kDa -
Additional sequence information
Recombinant full length Cyclin E1 (NM_001238)
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Preparation and Storage
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
This is a complex of Cdk3 and Cyclin E1 Co-expressed in a Baculovirus-infected Sf9 cell expression system.
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Images
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Functional Studies - Recombinant human Cyclin E1 + Cdk3 protein (Active) (ab196131)
Specific activity of ab196131 was determined to be 30 pmol/min/μg.
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SDS-PAGE - Recombinant human Cyclin E1 + Cdk3 protein (Active) (ab196131)10% SDS-PAGE analysis of ab196131 stained with Coomassie Blue.
Lane 1: Cdk3 Lower band and Cyclin E1 upper band
Lane 2: Protein Marker