Recombinant human ADAMTS5 protein
See all ADAMTS5 proteins and peptides

Aggrecanase activity of ab134432 is determined with recombinant aggrecan interglobular domain. ADAMTS4 hydrolyzes the aggrecanase site within this domain (peptide bond E₃₇₃-A₃₇₄ in Human aggrecan). The recombinant substrate is incubated at a concentration of 0.1 µM with ADAMTS5 in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 M CaCl2, 1 µM leupeptin, 1 µM pepstatin, 1 mM Pefabloc, 0.05 % Brij 35 for 15 min at 37°C. A series of ADAMTS5 dilutions ranging from 103 - to 105 -fold is tested. Substrate cleavage at the aggrecanase-site is estimated from the appearance of the hydrolysis fragment with the novel N terminus ARGSVIL. The fragment is quantified with two monoclonal antibodies, one directed against the neoepitope ARGSVIL, the other against the sequence C terminal from the neoepitope. Under the specified conditions the hydrolysis rate is > 0.5 nmoles hydrolyzed substrate/ min x ml ADAMTS5 preparation or > 5 nmoles hydrolyzed substrate/ min x mg.

>= 50 % SDS-PAGE.
ab134432 was determined to be >50% pure by SDS-PAGE.

Baculovirus infected insect cells

Protein fragment

No

Recombinant

Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

pH: 7.50
Preservative: 0.34% Imidazole
Constituents: 0.05% Brij, 0.05% Calcium chloride, 0.79% Tris HCl, 0.88% Sodium chloride

This product is an active protein and may elicit a biological response in vivo, handle with caution.