Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aggrecan antibody [6-B-4] - BSA and Azide free (ab255969)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [6-B-4] to Aggrecan - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Aggrecan antibody [6-B-4] - BSA and Azide free
    See all Aggrecan primary antibodies

  • Description

    Mouse monoclonal [6-B-4] to Aggrecan - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant human Aggrecan protein. IHC-P: Human cartilage sections.

  • General notes

    ab255969 is the carrier-free version of ab3778. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255969 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Aggrecan antibody [6-B-4] - BSA and Azide free (ab255969)
    Western blot - Anti-Aggrecan antibody [6-B-4] - BSA and Azide free (ab255969)

    This data was produced using the same clone but in a different buffer formulation, ab3778.

    ab3778 was shown to react with recombinant human Aggrecan protein in western blot. 

    Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3778 at 1µg/ml overnight at 4°C. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye®800CW) preabsorbed (ab216772) at a 1/20000 dilution for 1 hour at RT.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] - BSA and Azide free (ab255969)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] - BSA and Azide free (ab255969)

    This data was produced using the same clone but in a different buffer formulation, ab3778.

    Secreted staining of cartilage cells.

    Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] - BSA and Azide free (ab255969)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] - BSA and Azide free (ab255969)

    This data was produced using the same clone but in a different buffer formulation, ab3778.

    No staining in negative control tissue (human normal liver).

    Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Anti-Aggrecan antibody [6-B-4] - BSA and Azide free (ab255969)
    Anti-Aggrecan antibody [6-B-4] - BSA and Azide free (ab255969)

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