Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR037F] to TRAP220/MED1 - BSA free
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Human

Product name

Anti-TRAP220/MED1 antibody [BLR037F] - BSA free
See all TRAP220/MED1 primary antibodies
Description

Rabbit monoclonal [BLR037F] to TRAP220/MED1 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide within Human TRAP220/MED1 aa 1550-1581 (C terminal). The exact sequence is proprietary. NP_004765.2 and Gene ID 5469.
Database link: Q15648
General notes

This product is sold under License from Bethyl Laboratories, Inc.

ab272086 is the carrier-free version of ab243893. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR037F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)

TRAP220/MED1 was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243893 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243893 at 1/1000 dilution.

Lane 1: ab243893 IP in HeLa whole cell lysate.

Lane 2: Control IgG in HeLa whole cell lysate.

Detection: Chemiluminescence with an exposure time of 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243893).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)

Formalin-fixed, paraffin-embedded human small cell lung cancer tissue stained for TRAP220/MED1using ab243893 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243893).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)

Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for TRAP220/MED1 using ab243893 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243893).
Immunocytochemistry - Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)

Formalin-fixed, paraffin-embedded HCT 116 (human colorectal carcinoma cell line) cells labeling TRAF220/MED1 using ab243893 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243893).
Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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