Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)
Key features and details
- Rabbit polyclonal to TRAP220/MED1 (phospho T1457)
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRAP220/MED1 (phospho T1457) antibody
See all TRAP220/MED1 primary antibodies -
Description
Rabbit polyclonal to TRAP220/MED1 (phospho T1457) -
Host species
Rabbit -
Specificity
ab60950 detects endogenous levels of TRAP220/MED1 only when phosphorylated at threonine 1457. -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide derived from human TRAP220/MED1 around the phosphorylation site of threonine 1457 (A-Y-TP-P-Q).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab60950 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
Images
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All lanes : Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950) at 1/500 dilution
Lane 1 : HuvEc cell extracts
treated with Serum (20%, 30mins)
Lane 2 : HuvEc cell extracts
treated with Serum (20%, 30mins) with immunising peptide
Predicted band size: 180 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)ab60950 at 1/50 - 1/100 dilution staining TRAP220/MED1 in human heart by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide
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ab60950 at 1/500 - 1/1000 dilution staining TRAP220/MED1 in HeLa cells by Immunofluorescence.