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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)

Price and availability

301 536 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to TRAP220/MED1 (phospho T1457)
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TRAP220/MED1 (phospho T1457) antibody
    See all TRAP220/MED1 primary antibodies
  • Description

    Rabbit polyclonal to TRAP220/MED1 (phospho T1457)
  • Host species

    Rabbit
  • Specificity

    ab60950 detects endogenous levels of TRAP220/MED1 only when phosphorylated at threonine 1457.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human TRAP220/MED1 around the phosphorylation site of threonine 1457 (A-Y-TP-P-Q).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab60950 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)
    Western blot - Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)
    All lanes : Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950) at 1/500 dilution

    Lane 1 : HuvEc cell extracts
    treated with Serum (20%, 30mins)
    Lane 2 : HuvEc cell extracts
    treated with Serum (20%, 30mins) with immunising peptide

    Predicted band size: 180 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)
    ab60950 at 1/50 - 1/100 dilution staining TRAP220/MED1 in human heart by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide
  • Immunocytochemistry/ Immunofluorescence - Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)
    Immunocytochemistry/ Immunofluorescence - Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)
    ab60950 at 1/500 - 1/1000 dilution staining TRAP220/MED1 in HeLa cells by Immunofluorescence.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-TRAP220/MED1 (phospho T1457) antibody (ab60950)

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    Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] (ab181103)

    Applications: Dot, IHC-P, WB

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    Anti-TRAP220/MED1 (phospho T1457) antibody (ab182660)

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    Anti-TRAP220/MED1 antibody (ab245546)

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    Anti-TRAP220/MED1 antibody (ab64965)

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    Anti-TRAP220/MED1 antibody [BLR037F] - BSA free (ab272086)

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    Anti-TRAP220/MED1 antibody (ab84386)

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    Anti-TRAP220/MED1 antibody [BLR037F] (ab243893)

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