Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18937] to Hepcidin + Hepcidin-2
  • Suitable for: IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Hepcidin + Hepcidin-2 antibody [EPR18937]
    See all Hepcidin + Hepcidin-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR18937] to Hepcidin + Hepcidin-2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse Hepcidin recombinant fragment protein; Mouse Hepcidin-2 recombinant fragment protein; Mouse liver lysate. IHC-P: Mouse liver tissue. IHC-F: Mouse liver tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18937

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on hepatocytes of Mouse liver was observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)
    Western blot - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)
    All lanes : Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775) at 1/200 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Mouse kidney lysate
    Lane 5 : Mouse spleen lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 9 kDa
    Observed band size: 9 kDa


    Exposure time: 20 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Highly expressed in the liver and to a much lesser extent in the heart (PMID:11113132 and PMID:12729891)

  • Western blot - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)
    Western blot - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)
    All lanes : Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775) at 1/5000 dilution

    Lane 1 : Mouse Hepcidin recombinant fragment protein
    Lane 2 : Mouse Hepcidin-2 recombinant fragment protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 9 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time:

    Lane 1: 1 second.
    Lane 2: 2 seconds.

    The mouse recombinant fragment proteins contain aa24-83 with a GST-Tag and were made in-house.

  • Immunohistochemistry (Frozen sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)
    Immunohistochemistry (Frozen sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized frozen section of Mouse liver labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on hepatocytes of Mouse liver.

    Counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Mouse kidney. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Mouse spleen. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Rat liver. This could be because the ab does not XR with Rat hepcidin (77% identity between mouse and rat hapcidin sequences).

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human colon cancer. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)
    Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.