Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13837] to 53BP2/ASPP2/BBP
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-53BP2/ASPP2/BBP antibody [EPR13837]
    See all 53BP2/ASPP2/BBP primary antibodies

  • Description

    Rabbit monoclonal [EPR13837] to 53BP2/ASPP2/BBP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human 53BP2/ASPP2/BBP aa 50-150. The exact sequence is proprietary.
    Database link: Q13625

  • Positive control

    • WB: Rat and mouse brain lysates; MCF-7, Jurkat and Saos-2 cell lysates IHC-P: Human kidney, spleen and testis tissue; Rat and mouse liver tissue

  • General notes

    This product was previously labelled as 53BP2/ASPP2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13837

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)
    Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)
    All lanes : Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377) at 1/10000 dilution (Purified)

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 126 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in PMID: 14766226 

  • Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)
    Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)
    Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377) at 1/10000 dilution (Purified) + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 126 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in PMID: 14766226 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling 53BP2/ASPP2/BBP with purified ab181377 at 1/1000 dilution (0.15 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling 53BP2/ASPP2/BBP with purified ab181377 at 1/1000 dilution (0.15 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling 53BP2/ASPP2/BBP with purified ab181377 at 1/1000 dilution (0.15 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling 53BP2/ASPP2/BBP with ab181377 (unpurified) at a 1/100 dilution.

  • Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)
    Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)
    All lanes : Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377) at 1/10000 dilution (unpurified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : Saos-2 cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 126 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling 53BP2/ASPP2/BBP with ab181377 (unpurified) at a 1/100 dilution.

  • Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)
    Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

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