Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13837] to 53BP2/ASPP2/BBP
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-53BP2/ASPP2/BBP antibody [EPR13837]
See all 53BP2/ASPP2/BBP primary antibodies
Description

Rabbit monoclonal [EPR13837] to 53BP2/ASPP2/BBP
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human 53BP2/ASPP2/BBP aa 50-150. The exact sequence is proprietary.
Database link: Q13625
Positive control
- WB: Rat and mouse brain lysates; MCF-7, Jurkat and Saos-2 cell lysates IHC-P: Human kidney, spleen and testis tissue; Rat and mouse liver tissue
General notes
This product was previously labelled as 53BP2/ASPP2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13837
Isotype

IgG
Research areas

Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

All lanes : Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377) at 1/10000 dilution (Purified)

Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 126 kDa
Observed band size: 150 kDa
why is the actual band size different from the predicted?

The molecular weight observed is consistent with what has been described in PMID: 14766226
Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377) at 1/10000 dilution (Purified) + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 126 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?

The molecular weight observed is consistent with what has been described in PMID: 14766226
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling 53BP2/ASPP2/BBP with purified ab181377 at 1/1000 dilution (0.15 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling 53BP2/ASPP2/BBP with purified ab181377 at 1/1000 dilution (0.15 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling 53BP2/ASPP2/BBP with purified ab181377 at 1/1000 dilution (0.15 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling 53BP2/ASPP2/BBP with ab181377 (unpurified) at a 1/100 dilution.
Western blot - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

All lanes : Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377) at 1/10000 dilution (unpurified)

Lane 1 : Jurkat cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 126 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling 53BP2/ASPP2/BBP with ab181377 (unpurified) at a 1/100 dilution.
Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377)

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