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Anti-53BP2/ASPP2/BBP antibody (ab236448)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-53BP2/ASPP2/BBP antibody (ab236448)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to 53BP2/ASPP2/BBP
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-53BP2/ASPP2/BBP antibody
    See all 53BP2/ASPP2/BBP primary antibodies
  • Description

    Rabbit polyclonal to 53BP2/ASPP2/BBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human 53BP2/ASPP2/BBP (N terminal). The exact sequence is proprietary.
    Database link: Q13625

  • Positive control

    • WB: U-2 OS, mouse MEF and INS-1 cell lysates. IP: U-2 OS cell lysate. ICC/IF: U-2 OS cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP.
  • General notes

     This product was previously labelled as 53BP2/ASPP2

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53

Images

  • Western blot - Anti-53BP2/ASPP2/BBP antibody (ab236448)
    Western blot - Anti-53BP2/ASPP2/BBP antibody (ab236448)
    All lanes : Anti-53BP2/ASPP2/BBP antibody (ab236448) at 1/2000 dilution

    Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with control siRNA
    Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with 53BP2/ASPP2/BBP siRNA
    Lane 3 : Mouse MEF cell lysate
    Lane 4 : Rat INS-1 cell lysate

    Predicted band size: 126 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-53BP2/ASPP2/BBP antibody (ab236448)
    Immunocytochemistry/ Immunofluorescence - Anti-53BP2/ASPP2/BBP antibody (ab236448)

    2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP stained for 53BP2/ASPP2/BBP (red) using ab236448 at 1/2000 dilution in ICC/IF. GFP (green). Right panel shows the merged images.

  • Immunocytochemistry/ Immunofluorescence - Anti-53BP2/ASPP2/BBP antibody (ab236448)
    Immunocytochemistry/ Immunofluorescence - Anti-53BP2/ASPP2/BBP antibody (ab236448)

    2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP stained for 53BP2/ASPP2/BBP (red) using ab236448 at 1/2000 dilution in ICC/IF. GFP (green). Lower panel shows the merged images.

  • Immunoprecipitation - Anti-53BP2/ASPP2/BBP antibody (ab236448)
    Immunoprecipitation - Anti-53BP2/ASPP2/BBP antibody (ab236448)

    53BP2/ASPP2/BBP was immunoprecipitated from ~400 µg U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate with with 3 μl ab236448. Western blot was performed from the immunoprecipitate using ab236448 at 1/2000 dilution.

    Lane 1: U-2 OS cell lysate.

    Lane 2: Control IP in U-2 OS cell lysate.

    Lane 3: ab236448 IP in U-2 OS cell lysate.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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