Anti-53BP2/ASPP2/BBP antibody (ab236448)
Key features and details
- Rabbit polyclonal to 53BP2/ASPP2/BBP
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-53BP2/ASPP2/BBP antibody
See all 53BP2/ASPP2/BBP primary antibodies -
Description
Rabbit polyclonal to 53BP2/ASPP2/BBP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human 53BP2/ASPP2/BBP (N terminal). The exact sequence is proprietary.
Database link: Q13625 -
Positive control
- WB: U-2 OS, mouse MEF and INS-1 cell lysates. IP: U-2 OS cell lysate. ICC/IF: U-2 OS cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP.
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General notes
This product was previously labelled as 53BP2/ASPP2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-53BP2/ASPP2/BBP antibody (ab236448) at 1/2000 dilution
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with control siRNA
Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with 53BP2/ASPP2/BBP siRNA
Lane 3 : Mouse MEF cell lysate
Lane 4 : Rat INS-1 cell lysate
Predicted band size: 126 kDa
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2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP stained for 53BP2/ASPP2/BBP (red) using ab236448 at 1/2000 dilution in ICC/IF. GFP (green). Right panel shows the merged images.
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2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP stained for 53BP2/ASPP2/BBP (red) using ab236448 at 1/2000 dilution in ICC/IF. GFP (green). Lower panel shows the merged images.
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53BP2/ASPP2/BBP was immunoprecipitated from ~400 µg U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate with with 3 μl ab236448. Western blot was performed from the immunoprecipitate using ab236448 at 1/2000 dilution.
Lane 1: U-2 OS cell lysate.
Lane 2: Control IP in U-2 OS cell lysate.
Lane 3: ab236448 IP in U-2 OS cell lysate.