Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mesothelin antibody [EPR4509] - BSA and Azide free (ab248520)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4509] to Mesothelin - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Mesothelin antibody [EPR4509] - BSA and Azide free
    See all Mesothelin primary antibodies

  • Description

    Rabbit monoclonal [EPR4509] to Mesothelin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Mesothelin aa 250-400. The exact sequence is proprietary.
    Database link: Q13421

  • Positive control

    • IP: HeLa whole cell lysate.

  • General notes

    ab248520 is the carrier-free version of ab133489 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248520 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Mesothelin antibody [EPR4509] - BSA and Azide free (ab248520)
    Western blot - Anti-Mesothelin antibody [EPR4509] - BSA and Azide free (ab248520)
    All lanes : Anti-Mesothelin antibody [EPR4509] (ab133489) at 1/1000 dilution

    Lane 1 : Human fetal lung lysate
    Lane 2 : Human breast cancer lysate
    Lane 3 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 69 kDa



    This data was developed using ab133489, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR4509] - BSA and Azide free (ab248520)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR4509] - BSA and Azide free (ab248520)
    This data was developed using ab133489, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Mesothelin in paraffin embedded Human serous ovarian carcinoma tissue, using ab133489 at a dilution of 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Mesothelin antibody [EPR4509] - BSA and Azide free (ab248520)
    Immunoprecipitation - Anti-Mesothelin antibody [EPR4509] - BSA and Azide free (ab248520)

    This data was developed using ab133489, the same antibody clone in a different buffer formulation.
    Purified ab133489 at 1/40 dilution (2µg) immunoprecipitating Mesothelin in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab133489 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133489 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 40, 71 kDa
    The expression profile observed is consistent with what has been described in the literature (PMID: 16857795; PMID: 17332303).

  • Anti-Mesothelin antibody [EPR4509] - BSA and Azide free (ab248520)
    Anti-Mesothelin antibody [EPR4509] - BSA and Azide free (ab248520)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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