Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Rad50 antibody [EPR3466(2)] (ab124682)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3466(2)] to Rad50
Suitable for: Flow Cyt, ICC/IF, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Rad50 antibody [EPR3466(2)]
See all Rad50 primary antibodies
Description

Rabbit monoclonal [EPR3466(2)] to Rad50
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Rad50 aa 1250-1350. The exact sequence is proprietary.
Positive control
- K562, MOLT4, Jurkat and MCF7 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3466(2)
Isotype

IgG
Research areas

Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)

All lanes : Anti-Rad50 antibody [EPR3466(2)] (ab124682) at 1/5000 dilution (purified)

Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 154 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Flow Cytometry - Anti-Rad50 antibody [EPR3466(2)] (ab124682)

Flow Cytometry analysis of U-2 OS (Human bone osteosarcoma epithelial cell) cells labeling Rad50 with purified ab124682 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-Rad50 antibody [EPR3466(2)] (ab124682)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Rad50 with Purified ab124682 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^®594) 1:200 . ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)

All lanes : Anti-Rad50 antibody [EPR3466(2)] (ab124682) at 1/1000 dilution (purified)

Lane 1 : Human fetal brain lysates
Lane 2 : Mouse thymus lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 154 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)

Anti-Rad50 antibody [EPR3466(2)] (ab124682) at 1/1000 dilution (purified) + Rat testis lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 154 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Rad50 antibody [EPR3466(2)] (ab124682) This image is courtesy of an anonymous Abreview

Unpurified ab124682 staining Rad50 in the U2OS cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 5% BSA for 60 minutes. Samples were incubated with undiluted primary antibody for 2 hours. An undiluted FITC conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
See Abreview
Western blot - Anti-Rad50 antibody [EPR3466(2)] (ab124682)

All lanes : Anti-Rad50 antibody [EPR3466(2)] (ab124682) at 1/1000 dilution (unpurified)

Lane 1 : K562 cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 154 kDa
Observed band size: 150 kDa
why is the actual band size different from the predicted?
Anti-Rad50 antibody [EPR3466(2)] (ab124682)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com