Anti-Rad50 antibody - N-terminal (ab228935)
Key features and details
- Rabbit polyclonal to Rad50 - N-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Rad50 antibody - N-terminal
See all Rad50 primary antibodies -
Description
Rabbit polyclonal to Rad50 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow
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Immunogen
Recombinant fragment within Human Rad50 (N terminal). The exact sequence is proprietary.
Database link: Q92878 -
Positive control
- WB: HeLa whole cell and nuclear extracts. Neuro-2a, NT2/D1, PC-12 and GL261 whole cell lysate. IHC-P: Human lung carcinoma tissue. IP: NT2/D1 whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rad50 antibody - N-terminal (ab228935)All lanes : Anti-Rad50 antibody - N-terminal (ab228935) at 1/4000 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 2 : Rad50 shRNA transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 154 kDa5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody - N-terminal (ab228935)Paraffin-embedded human lung carcinoma tissue stained for Rad50 with ab228935 at a 1/250 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-Rad50 antibody - N-terminal (ab228935)Rad50 was immunoprecipitated from NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate with 4 µg ab228935. Western blot was performed from the immunoprecipitate using ab228935 at a 1/500 dilution.
Lane 1: 50 µg NT2/D1 whole cell lysate.
Lane 2: Control with 4 µg preimmune Rabbit IgG.
Lane 3: ab228935 in NT2/D1 whole cell lysate.
5% SDS-PAGE
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Western blot - Anti-Rad50 antibody - N-terminal (ab228935)All lanes : Anti-Rad50 antibody - N-terminal (ab228935) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 154 kDa5% SDS-PAGE
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Western blot - Anti-Rad50 antibody - N-terminal (ab228935)All lanes : Anti-Rad50 antibody - N-terminal (ab228935) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : GL261 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 154 kDa5% SDS-PAGE
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Western blot - Anti-Rad50 antibody - N-terminal (ab228935)Anti-Rad50 antibody - N-terminal (ab228935) at 1/5000 dilution + NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 154 kDa5% SDS-PAGE
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Western blot - Anti-Rad50 antibody - N-terminal (ab228935)Anti-Rad50 antibody - N-terminal (ab228935) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extracts at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 154 kDa5% SDS-PAGE
