Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

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Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20968] to Rad50 - ChIP Grade
Suitable for: WB, ChIP, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Rad50 antibody [EPR20968] - ChIP Grade
See all Rad50 primary antibodies
Description

Rabbit monoclonal [EPR20968] to Rad50 - ChIP Grade
Host species

Rabbit

Tested Applications & Species

Application	Species
ChIP	Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal brain and kidney lysates; K562, HeLa, HEK-293T, HT-29, NIH/3T3, ES-D3, C6, RAW 264.7 and PC-12 cell lysates; Mouse kidney lysate; Rat brain and spleen lysate. IHC-P: Human breast and breast carcinoma tissue; Mouse and rat testis tissue. ChIP: Chromatin prepared from HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20968
Isotype

IgG
Research areas

Western blot - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

All lanes : Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019) at 1/1000 dilution

Lane 1 : Mouse kidney lysate at 10 µg
Lane 2 : Rat brain lysate at 10 µg
Lane 3 : Rat spleen lysate at 10 µg
Lane 4 : C6 (rat glial tumor cell line) whole cell lysate at 20 µg
Lane 5 : RAW 364.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 153 kDa
Observed band size: 154 kDa
why is the actual band size different from the predicted?

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: Lanes 1-3: 3 minutes; Lanes 4-6: 41 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID: 26068589).
ChIP - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with 1% methymethanesulfonate (MMS) according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab208019 (red), and 20 µl of Protein A/G sepharose beads. 2μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach). rDNA primers used are located in the region of ribosomal gene loci following the publication PMID:21029860(rDNA 1: rDNA 13017F/13068R ; rDNA 2 : rDNA 1125F/1201R ; rDNA 3 : rDNA 30409F/30566R).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Rad50 with ab208019 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human breast tissue (PMID: 15509680) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Rad50 with ab208019 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in rat testis is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Rad50 with ab208019 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse testis (PMID: 10908350) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Rad50 with ab208019 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human breast carcinoma (PMID: 24642965) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

All lanes : Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019) at 1/1000 dilution

Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lane 6 : ES-D3 (mouse embryonic multipotent stem Cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 153 kDa

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: Lanes 1-4: 8 seconds; Lanes 5-6: 24 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID: 26068589).
Western blot - Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

All lanes : Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 153 kDa

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: Line 1: 3 minutes; Line 2: 41 seconds.
Anti-Rad50 antibody [EPR20968] - ChIP Grade (ab208019)

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