Rap1 Activation Assay Kit (ab212011)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Rap1 Activation Assay Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
Rap1 Activation Assay Kit (ab212011) uses RalGDS RBD Agarose beads to selectively isolate and pull-down the active form of Rap from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rap is detected by western blot analysis using an anti-Rap1 polyclonal antibody, which reacts with the human, mouse and rat Rap1A and Rap1B proteins.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
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Notes
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Rap1 (Ras-related protein 1), a 21 kDa protein of the Ras superfamily, exhibits 60% identity to Rap2. Rap2 regulates a variety of biological response pathways that include cell migration, cell adhesion, and embryonic blood vessel formation. Like other small GTPases, Rap1 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rap1 bind specifically to the Rap-binding domain (RBD) of RalGDS to control downstream signaling cascades.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 20 tests 100X GDP 1 x 50µl 100X GTPyS 1 x 50µl 5X Assay/Lysis Buffer 1 x 30ml ab211179 - RalGDS RBD Agarose Beads 1 x 800µl Anti-Rap1 Goat polyclonal antibody 1 x 40µl GTPase immunoblot Positive Control 1 x 100µl -
Research areas
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Function
Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes. -
Tissue specificity
Ubiquitous. Highly expressed. -
Sequence similarities
Belongs to the RAP1 family.
Contains 1 BRCT domain.
Contains 1 Myb-like domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Cytoplasm. Chromosome. Chromosome > telomere. Associates with chromosomes, both at telomeres and in extratelomeric sites. Also exists as a cytoplasmic form, where it associates with the IKK complex. - Information by UniProt
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Alternative names
- Dopamine receptor interacting protein 5
- Dopamine receptor-interacting protein 5
- DRIP 5
see all -
Database links
- Entrez Gene: 54386 Human
- Entrez Gene: 57321 Mouse
- Entrez Gene: 307861 Rat
- Omim: 605061 Human
- SwissProt: Q9NYB0 Human
- SwissProt: Q91VL8 Mouse
- SwissProt: Q5EAN7 Rat
- Unigene: 301419 Human
see all
Images
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Rap1 Activation Assay Kit (ab212011)
Rap1 positive control (10 µL of NIH3T3 cell lysate at 0.75 mg/mL) detected by immunoblot.
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Rap1 Activation Assay Kit (ab212011)Rap1 activation assay. Lane 1: NIH 3T3 cell lysate loaded with GDP and incubated with RalGDS RBD Agarose beads. Lane 2: NIH 3T3 cell lysate loaded with GTPγS and incubated with RalGDS RBD Agarose beads.
