Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12404] to NHP2 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NHP2 antibody [EPR12404] - BSA and Azide free
    See all NHP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12404] to NHP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250201 is the carrier-free version of ab180498. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250201 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)
    Western blot - Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)
    All lanes : Anti-NHP2 antibody [EPR12404] (ab180498) at 1/2000 dilution

    Lane 1 : K562 lysate
    Lane 2 : Jurkat lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa



    This data was developed using ab180498, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)
    This data was developed using ab180498, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling NHP2 with ab180498 at 1/500 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG and counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)
    Flow Cytometry - Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)
    This data was developed using ab180498, the same antibody clone in a different buffer formulation.Flow Cytometric analysis of K562 cells (2% paraformaldehyde-fixed) labeling NHP2 with ab180498 at 1/20 (red) compared to a Rabbit monoclonal IgG negative control antibody (green) followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.
  • Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)
    Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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