Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)
![Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)](https://www.abcam.com/ps/products/250/ab250201/Images/ab250201-405663-anti-nhp2-antibody-epr12404-bsa-and-azide-free-western-blot.jpg "Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12404] to NHP2 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NHP2 antibody [EPR12404] - BSA and Azide free
See all NHP2 primary antibodies -
Description
Rabbit monoclonal [EPR12404] to NHP2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250201 is the carrier-free version of ab180498. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250201 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12404 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)All lanes : Anti-NHP2 antibody [EPR12404] (ab180498) at 1/2000 dilution
Lane 1 : K562 lysate
Lane 2 : Jurkat lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDaThis data was developed using ab180498, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)](https://www.abcam.com/ps/products/250/ab250201/Images/ab250201-2-anti-nhp2-antibody-epr12404-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)This data was developed using ab180498, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling NHP2 with ab180498 at 1/500 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG and counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Flow Cytometry - Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)](https://www.abcam.com/ps/products/250/ab250201/Images/ab250201-1-anti-nhp2-antibody-epr12404-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)This data was developed using ab180498, the same antibody clone in a different buffer formulation.Flow Cytometric analysis of K562 cells (2% paraformaldehyde-fixed) labeling NHP2 with ab180498 at 1/20 (red) compared to a Rabbit monoclonal IgG negative control antibody (green) followed by Goat anti rabbit IgG (FITC) at 1/75 dilution. -
![Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)](https://www.abcam.com/ps/products/250/ab250201/Images/ab250201-4-benefits-of-recombinant-antibodies.png)
Anti-NHP2 antibody [EPR12404] - BSA and Azide free (ab250201)
