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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins

Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP1629Y] to Cytokeratin 8 (phospho S23)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y]
    See all Cytokeratin 8 primary antibodies
  • Description

    Rabbit monoclonal [EP1629Y] to Cytokeratin 8 (phospho S23)
  • Host species

    Rabbit
  • Specificity

    ab76584 detects Cytokeratin 8 phosphorylated on serine 23.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding serine 23 of human Cytokeratin 8

  • Positive control

    • HeLa cell lysates, untreated or treated with Calyculin; human gastric adenocarcinoma and human breast adenocarcinoma tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP1629Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)

    ab76584, at a 1/100 dilution, staining Cytokeratin 8 in paraffin embedded human gastric adenocarcinoma (image 1) and human breast adenocarcinoma (image 2) tissues by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)
    Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)
    All lanes : Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584) at 1/50000 dilution

    Lane 1 : HeLa cell lysate, untreated
    Lane 2 : HeLa cell lysate treated with Calyculin

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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