Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10635(B)] to Matrin 3 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free
    See all Matrin 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR10635(B)] to Matrin 3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240123 is the carrier-free version of ab151714. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240123 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)
    Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Matrin 3 with purified ab151714 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 μg/mL) - Microtubule Marker (Alexa Fluor® 594) at 1/200. DAPI (blue) was used as a nuclear counterstain. Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151714).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

    Immunohistochemical analysis of Paraffin-embedded rat liver tissue sections labeling Matrin 3 with purified ab151714 at a dilution of 1/2000 (0.7 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using citrate Buffer, pH 6.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151714).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue sections labeling Matrin 3 with purified ab151714 at a dilution of 1/2000 (0.7 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using citrate Buffer, pH 6.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151714).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

    Immunohistochemical analysis of paraffin-embedded human colon tissue sections labeling Matrin 3 with purified ab151714 at a dilution of 1/2000 (0.7 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using citrate Buffer, pH 6.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151714).

  • Flow Cytometry - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)
    Flow Cytometry - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

    Flow cytometry analysis of 4% paraformaldehyde fixed HepG2 (human hepatocellular carcinoma) cells labeling Matrin 3 with purified ab151714 at a dilution of 1/220. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)
    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151714).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Matrin 3 with unpurified ab151714 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151714).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Matrin 3 with unpurified ab151714 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151714).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)
    Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

    Immunofluorescent analysis of HepG2 cells labeling Matrin 3 with unpurified ab151714 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151714).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

    Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using unpurified ab151714 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151714).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

    Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using unpurified ab151714 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151714).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

    Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using unpurified ab151714 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151714).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)
    Anti-Matrin 3 antibody [EPR10635(B)] - BSA and Azide free (ab240123)

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