Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10635(B)] to Matrin 3
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Matrin 3 antibody [EPR10635(B)]
See all Matrin 3 primary antibodies
Description

Rabbit monoclonal [EPR10635(B)] to Matrin 3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

within Human Matrin 3. The exact sequence is proprietary.
Database link: P43243
Positive control
- HEK293T, K562, HeLa and HepG2 whole cell lysate (ab7900); Human brain tissue, Human colon tissue and Human breast carcinoma tissue; Mouse and rat liver tissue, Mouse and Rat brain tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10635(B)
Isotype

IgG
Research areas

Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

All lanes : Anti-Matrin 3 antibody [EPR10635(B)] (ab151714) at 1/10000 dilution (purified)

Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 95 kDa
Observed band size: 125 kDa
why is the actual band size different from the predicted?

Blocking/Diluting buffer 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

Immunohistochemical analysis of paraffin-embedded human colon tissue sections labeling Matrin 3 with purified ab151714 at a dilution of 1/2000 (0.7 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using citrate Buffer, pH 6.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Matrin 3 with purified ab151714 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 μg/mL) - Microtubule Marker (Alexa Fluor^® 594) at 1/200. DAPI (blue) was used as a nuclear counterstain. Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Western blot - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

All lanes : Anti-Matrin 3 antibody [EPR10635(B)] (ab151714) at 1/1000 dilution (unpurified)

Lane 1 : HEK293T cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 95 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

Immunohistochemical analysis of Paraffin-embedded rat liver tissue sections labeling Matrin 3 with purified ab151714 at a dilution of 1/2000 (0.7 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using citrate Buffer, pH 6.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue sections labeling Matrin 3 with purified ab151714 at a dilution of 1/2000 (0.7 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using citrate Buffer, pH 6.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Flow Cytometry - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

Flow cytometry analysis of 4% paraformaldehyde fixed HepG2 (human hepatocellular carcinoma) cells labeling Matrin 3 with purified ab151714 at a dilution of 1/220. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Matrin 3 with unpurified ab151714 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Matrin 3 with unpurified ab151714 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

Immunofluorescent analysis of HepG2 cells labeling Matrin 3 with unpurified ab151714 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using unpurified ab151714 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using unpurified ab151714 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using unpurified ab151714 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Matrin 3 antibody [EPR10635(B)] (ab151714)

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