Anti-Matrin 3 antibody (ab84422)
Key features and details
- Rabbit polyclonal to Matrin 3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Matrin 3 antibody
See all Matrin 3 primary antibodies -
Description
Rabbit polyclonal to Matrin 3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan, Bat
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Immunogen
Synthetic peptide designed within residues: GFYCKLCSLF YTNEEVAKNT HCSSLPHYQK LKKFLNKLAE ERRQKKET, corresponding to C terminal amino acids 800-847 of Human Matrin 3 (NP_061322.2)
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Positive control
- Human: breast carcinoma, colon carcinoma, laryngeal squamous cell carcinoma, linitis plastica stomach cancer, lung adenocarcinoma, metastatic lymph node, non-small cell lung cancer, ovarian carcinoma, pancreatic islet cell tumor, prostate carcinoma, skin basal cell carcinoma, skin squamous cell carcinoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma. Mouse: squamous cell carcinoma.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (ab84422)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon adenocarcinoma using 1/250 ab84422, followed by DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrin 3 antibody (ab84422)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse squamous cell carcinoma using 1/250 ab84422, followed by DAB staining. -
Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody (ab84422)Detection of Human Matrin 3 by Immunofluorescence.
Sample: FFPE section of human breast carcinoma.Antibody: ab84422 used at a dilution of 1/100.
Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500. -
Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody (ab84422)Detection of Human Matrin 3 by Immunofluorescence.
Sample: FFPE section of Human breast carcinoma.Antibody: ab84422 used at a dilution of 1/100.
Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500. -
Immunocytochemistry/ Immunofluorescence - Anti-Matrin 3 antibody (ab84422) Image courtesy of Leilani Beltran by Abreview.ab84422 staining Matrin 3 in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0.5% NP-40, blocked with 1% BSA for 1 hour a room temperature, then incubated with ab84422 at a 1/100 dilution for 1 hour. The secondary antibody used was an Alexa-Fluor 488 conjugate donkey polyclonal, used at a 1/1000 dilution.
