Human TOP2B (Topoisomerase II beta) knockout HEK-293T cell line (ab266340)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Kidney -
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12 -
Antibiotic resistance
Puromycin 1.00µg/ml -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Research areas
Images
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Western blot - Human TOP2B knockout HEK293T cell line (ab266340)All lanes : Anti-Topoisomerase II beta/TOP2B antibody (ab125297)
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : TOP2B knockout HEK-293T cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 183 kDa
Observed band size: 183 kDaLanes 1- 2: Merged signal (red and green). Green - ab125297 observed at 183 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.
ab125297 was shown to react with Topoisomerase II beta/TOP2B in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266340 (knockout cell lysate ab257286) was used. Wild-type HEK-293T and TOP2B knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab125297 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 µg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human TOP2B knockout HEK293T cell line (ab266340)Allele-1: 17 bp deletion in exon 3
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Sanger Sequencing - Human TOP2B knockout HEK293T cell line (ab266340)Allele-2: 13 bp deletion in exon 3.
