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Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16224-32] to KIF5C - BSA and Azide free
  • Suitable for: WB, ICC, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free
    See all KIF5C primary antibodies

  • Description

    Rabbit monoclonal [EPR16224-32] to KIF5C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251167 is the carrier-free version of ab193352.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)
    Immunocytochemistry - Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)
    This data was developed using ab193352, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed SH-SY5Y cells labeling KIF5C using ab193352 at a 1/150 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.
  • Western blot - Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)
    Western blot - Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)
    All lanes : Anti-KIF5C antibody [EPR16224-32] (ab193352) at 1/10000 dilution

    Lane 1 : Neuro-2a lysate
    Lane 2 : Human fetal brain tissue lysate
    Lane 3 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 109 kDa



    This data was developed using ab193352, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)
    Western blot - Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)
    All lanes : Anti-KIF5C antibody [EPR16224-32] (ab193352) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : C6 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 109 kDa



    This data was developed using ab193352, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)
    This data was developed using ab193352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human testis tissue sections labeling KIF5C using ab193352 at a 1/300 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)
    This data was developed using ab193352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded mouse cerebral cortex tissue sections labeling KIF5C using ab193352 at a 1/300 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)
    Anti-KIF5C antibody [EPR16224-32] - BSA and Azide free (ab251167)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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