Anti-Rad50 antibody (ab228886)
Key features and details
- Rabbit polyclonal to Rad50
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Rad50 antibody
See all Rad50 primary antibodies -
Description
Rabbit polyclonal to Rad50 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow
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Immunogen
Recombinant fragment within Human Rad50 (internal sequence). The exact sequence is proprietary.
Database link: Q92878 -
Positive control
- WB: HeLa whole cell extract; NT2/D1 whole cell lysate; rat testis tissue extract. IHC-P: Human colon carcinoma tissue. IP: NT2/D1 whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Rad50 antibody (ab228886)Anti-Rad50 antibody (ab228886) at 1/5000 dilution + Mouse testis tissue extract at 50 µg
Developed using the ECL technique.
Predicted band size: 154 kDa5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad50 antibody (ab228886)Paraffin-embedded human colon carcinoma tissue stained for Rad50 using ab228886 at 1/250 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-Rad50 antibody (ab228886)Rad50 protein was immunoprecipitated from 50 μg NT2/D1 (human embryonal testis carcinoma cell line) whole cell extract with 4 μg of ab228886. Western blot was performed from the immunoprecipitate using ab228886 at 1/500 dilution.
Lane 1: NT2/D1 whole cell extract 50 μg (input).
Lane 2: 4 μg preimmune rabbit IgG instead of ab228886 in NT2/D1 whole cell extract.
Lane 3: ab228886 in NT2/D1 whole cell extract.5% SDS-PAGE
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Western blot - Anti-Rad50 antibody (ab228886)Anti-Rad50 antibody (ab228886) at 1/1000 dilution + Rat testis tissue extract at 50 µg
Developed using the ECL technique.
Predicted band size: 154 kDa5% SDS-PAGE
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Western blot - Anti-Rad50 antibody (ab228886)Anti-Rad50 antibody (ab228886) at 1/5000 dilution + NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 154 kDa5% SDS-PAGE gel.
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Western blot - Anti-Rad50 antibody (ab228886)All lanes : Anti-Rad50 antibody (ab228886) at 1/2000 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 2 : Rad50 shRNA transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 154 kDa5% SDS-PAGE gel.
