Anti-Mre11 antibody (ab33125)
Key features and details
- Rabbit polyclonal to Mre11
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Mre11 antibody
See all Mre11 primary antibodies -
Description
Rabbit polyclonal to Mre11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide corresponding to Human Mre11 aa 600-700 conjugated to keyhole limpet haemocyanin.
(Peptide available asab33124) -
Positive control
- Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate; A431 (Human epithelial carcinoma cell line) Whole Cell Lysate;
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab33125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). IP Use at an assay dependent concentration. Target
-
Function
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. -
Involvement in disease
Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course. -
Sequence similarities
Belongs to the MRE11/RAD32 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
-
Database links
- Entrez Gene: 4361 Human
- Entrez Gene: 17535 Mouse
- Entrez Gene: 64046 Rat
- Omim: 600814 Human
- SwissProt: P49959 Human
- SwissProt: Q61216 Mouse
- SwissProt: Q9JIM0 Rat
- Unigene: 192649 Human
see all -
Alternative names
- AT like disease antibody
- Ataxia telangiectasia disorder like antibody
- ATLD antibody
see all
Images
-
Western blot - Anti-Mre11 antibody (ab33125)All lanes : Anti-Mre11 antibody (ab33125) at 1 µg/ml
Lane 1 :Jurkat whole cell lysate (ab7899)
Lane 2 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 81 kDa
Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-Mre11 antibody (ab33125)Mre11 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to Mre11 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33125.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 81kDa: Mre11 -
Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody (ab33125)This image is courtesy of an Abreview submitted by Dr Kirk McManusab33125 (1/200) stainning Mre11 in assynchronous HeLa cells (green). Cells were fixed using paraformaldehyde and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.
Protocols
Datasheets and documents
References (8)
ab33125 has been referenced in 8 publications.
- Bai Y et al. C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex. Mol Cell 75:1299-1314.e6 (2019). PubMed: 31353207
- Eryilmaz M et al. Localization Microscopy Analyses of MRE11 Clusters in 3D-Conserved Cell Nuclei of Different Cell Lines. Cancers (Basel) 10:N/A (2018). Human . PubMed: 29361783
- Forment JV et al. Genome-wide genetic screening with chemically mutagenized haploid embryonic stem cells. Nat Chem Biol 13:12-14 (2017). PubMed: 27820796
- Mariggiò G et al. Kaposi Sarcoma Herpesvirus (KSHV) Latency-Associated Nuclear Antigen (LANA) recruits components of the MRN (Mre11-Rad50-NBS1) repair complex to modulate an innate immune signaling pathway and viral latency. PLoS Pathog 13:e1006335 (2017). WB ; Human . PubMed: 28430817
- Chang L et al. Targeting Rad50 sensitizes human nasopharyngeal carcinoma cells to radiotherapy. BMC Cancer 16:190 (2016). WB, IP, ICC/IF ; Human . PubMed: 26951044
- Ahrabi S et al. A role for human homologous recombination factors in suppressing microhomology-mediated end joining. Nucleic Acids Res 44:5743-57 (2016). WB ; Human . PubMed: 27131361
- Davies OR et al. CtIP tetramer assembly is required for DNA-end resection and repair. Nat Struct Mol Biol 22:150-157 (2015). PubMed: 25558984
- Mondal NK et al. Micronucleus formation, DNA damage and repair in premenopausal women chronically exposed to high level of indoor air pollution from biomass fuel use in rural India. Mutat Res 697:47-54 (2010). ICC ; Human . PubMed: 20156583
Images
-
Western blot - Anti-Mre11 antibody (ab33125)All lanes : Anti-Mre11 antibody (ab33125) at 1 µg/ml
Lane 1 :Jurkat whole cell lysate (ab7899)
Lane 2 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 81 kDa
Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-Mre11 antibody (ab33125)Mre11 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to Mre11 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33125.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 81kDa: Mre11 -
Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody (ab33125) This image is courtesy of an Abreview submitted by Dr Kirk McManusab33125 (1/200) stainning Mre11 in assynchronous HeLa cells (green). Cells were fixed using paraformaldehyde and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.
