Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PINK1 antibody [N4/15] (ab186303)

Key features and details

  • Mouse monoclonal [N4/15] to PINK1
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PINK1 antibody [N4/15]
    See all PINK1 primary antibodies

  • Description

    Mouse monoclonal [N4/15] to PINK1

  • Host species

    Mouse

  • Specificity

    >30% identity with DMPK.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human PINK1 aa 100-500. NP_115785.1.
    Database link: Q9BXM7

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat brain lysate. ICC/IF: SK-N-BE cells.

  • General notes

    The clone number has been updated from S4-15 to N4/15, both clone numbers name the same antibody clone.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [N4/15] (ab186303)
    Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [N4/15] (ab186303)

    SK-N-BE (Human neuroblastoma cell line) cells stained for PINK1 using ab186303 at a dilution of 1/100 (1 hour at RT) in ICC/IF.

    Cells were fixed in 4% formaldehyde for 15 minutes at RT.

    Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 1 hour at RT.

  • Western blot - Anti-PINK1 antibody [N4/15] (ab186303)
    Western blot - Anti-PINK1 antibody [N4/15] (ab186303)
    Anti-PINK1 antibody [N4/15] (ab186303) at 1/200 dilution + Rat brain lysate at 15 µg

    Secondary
    Goat Anti-Mouse IgG: HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 63 kDa


    Exposure time: 6 minutes


    Block: 2% BSA and 2% skimmed milk in 1X TBST.

    Incubated with primary antibody for 16 hours at 4°C.

    Incubated with secondary antibody for 1 hour at RT.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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